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- KD/KO Validated
GM-CSF Polyclonal antibody
GM-CSF Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17762-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NK-92 cells |
| Positive IHC detected in | human tonsillitis tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 9 publications below |
| IF | See 5 publications below |
Product Information
17762-1-AP targets GM-CSF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GM-CSF fusion protein Ag12142 相同性解析による交差性が予測される生物種 |
| Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Calculated molecular weight | 144 aa, 16 kDa |
| Observed molecular weight | 15kDa,18 kDa ,22 kDa |
| GenBank accession number | BC108724 |
| Gene symbol | GM-CSF |
| Gene ID (NCBI) | 1437 |
| RRID | AB_2276718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GM-CSF antibody 17762-1-AP | Download protocol |
| IHC protocol for GM-CSF antibody 17762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hematol Oncol FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion | ||
J Exp Med Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma. | ||
J Am Soc Nephrol Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury. | ||
Acta Pharmacol Sin Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching | ||
Oncogene CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer. | ||
Int J Mol Sci Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice. |
