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  • Featured Product
  • KD/KO Validated

CSF2RA/CD116 Polyclonal antibody

CSF2RA/CD116 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18307-1-AP

Synonyms

CD116, GMCSFR alpha, GMCSFR, GM CSF R alpha, CSF2RA

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CSF2RA/CD116 Polyclonal antibody (18307-1-AP)

    WB analysis of THP-1 using 18307-1-AP

    WB result of CSF2RA/CD116 antibody (18307-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSF2RA/CD116 transfected THP-1 cells.

  • Western Blot (WB) analysis of various lysates using CSF2RA/CD116 Polyclonal antibody (18307-1-AP)

    WB analysis using 18307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSF2RA/CD116 Polyclonal antibody (18307-1-AP)

    IHC staining of human placenta using 18307-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSF2RA/CD116 Polyclonal antibody (18307-1-AP)

    IHC staining of human placenta using 18307-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18307-1-AP targets CSF2RA/CD116 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Calculated molecular weight 47 kDa
Observed molecular weight55 kDa
GenBank accession numberBC071835
Gene symbol CD116
Gene ID (NCBI) 1438
RRIDAB_2229990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

Protocols

Product Specific Protocols
WB protocol for CSF2RA/CD116 antibody 18307-1-APDownload protocol
IHC protocol for CSF2RA/CD116 antibody 18307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols