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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using CSGALNACT1 Polyclonal antibody (26913-1-AP)

    WB analysis of PC-3 using 26913-1-AP (same clone as 26913-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26913-1-AP (CSGALNACT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26913-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CSGALNACT1 Polyclonal antibody (26913-1-AP)

    WB analysis of MCF-7 using 26913-1-AP (same clone as 26913-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26913-1-AP (CSGALNACT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26913-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using CSGALNACT1 Polyclonal antibody (26913-1-AP)

    IP experiment of PC-3 using 26913-1-AP (same clone as 26913-1-PBS)

    IP result of anti-CSGALNACT1 (IP:26913-1-AP, 4ug; Detection:26913-1-AP 1:1000) with PC-3 cells lysate 3200ug. This data was developed using the same antibody clone with 26913-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26913-1-PBS targets CSGALNACT1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSGALNACT1 fusion protein Ag25455 相同性解析による交差性が予測される生物種
Full Name chondroitin sulfate N-acetylgalactosaminyltransferase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61-68 kDa
GenBank accession numberBC060772
Gene Symbol CSGALNACT1
Gene ID (NCBI) 55790
RRIDAB_2880682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDX6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CSGalNAcT-1 (chondroitin sulfate N-acetylgalactosaminyltransferase-1), catalyzing the transfer of a GalNAc residue onto the nonreducing end of GlcA, is a key glycosyltransferase for chondroitin sulfate (CS) biosynthesis in cartilage. Abnormal expression of CSGalNAcT-1 in cartilage has been linked to diseases like osteoarthritis.