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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSK Monoclonal antibody, PBS Only

CSK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66115-1-PBS

Synonyms

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of RAW 264.7 using 66115-1-Ig (same clone as 66115-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of NIH/3T3 using 66115-1-Ig (same clone as 66115-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of HeLa using 66115-1-Ig (same clone as 66115-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CSK Monoclonal antibody (66115-1-Ig)

    IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    Immunofluorescent analysis of A431 cells using 66115-1-Ig (CSK antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66115-1-PBS targets CSK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CSK fusion protein Ag12103 相同性解析による交差性が予測される生物種
Full Name c-src tyrosine kinase
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC106073
Gene symbol CSK
Gene ID (NCBI) 1445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).