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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSK Monoclonal antibody, PBS Only

CSK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66115-1-PBS

Synonyms

Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of RAW 264.7 using 66115-1-Ig (same clone as 66115-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of NIH/3T3 using 66115-1-Ig (same clone as 66115-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of HeLa using 66115-1-Ig (same clone as 66115-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CSK Monoclonal antibody (66115-1-Ig)

    IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSK antibody (66115-1-Ig, Clone: 3B12C5 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66115-1-PBS targets CSK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CSK fusion protein Ag12103 相同性解析による交差性が予測される生物種
Full Name c-src tyrosine kinase
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC106073
Gene symbol CSK
Gene ID (NCBI) 1445
RRIDAB_2881514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).