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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30498-1-AP

Synonyms

Beta casein, CASB, casein beta, CSN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSN2 Polyclonal antibody (30498-1-AP)

    WB analysis using 30498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CSN2 Polyclonal antibody (30498-1-AP)

    WB analysis using 30498-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSN2 Polyclonal antibody (30498-1-AP)

    IHC staining of human breast cancer using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using CSN2 Polyclonal antibody (30498-1-AP)

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using CSN2 Polyclonal antibody (30498-1-AP)

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HT-29 cells, NIH/3T3 cells, mouse liver tissue, mouse testis tissue, DU 145 cells, rat liver tissue
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30498-1-AP targets CSN2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSN2 fusion protein Ag27943 相同性解析による交差性が予測される生物種
Full Name casein beta
Observed molecular weight 60 kDa
GenBank accession numberBC096194
Gene symbol CSN2
Gene ID (NCBI) 1447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSN2 is also named as Beta-casein and CASB.The β-casein is the most abundant protein in camel milk, and is one of the most important milk protein fractions  (PMID: 24973699).

Protocols

Product Specific Protocols
WB protocol for CSN2 antibody 30498-1-APDownload protocol
IHC protocol for CSN2 antibody 30498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols