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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSNK1A1 Monoclonal antibody

CSNK1A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2E7

Cat no : 68434-1-Ig

Synonyms

CSNK, CKI-alpha, CKI alpha, CK1α, CK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK1A1 Monoclonal antibody (68434-1-Ig)

    WB analysis using 68434-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68434-1-Ig (CSNK1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68434-1-Ig targets CSNK1A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name casein kinase 1, alpha 1
Calculated molecular weight 42 kDa
Observed molecular weight30-40 kDa
GenBank accession numberNM_001892
Gene symbol CSNK1A1
Gene ID (NCBI) 1452
RRIDAB_3073926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Protocols

Product Specific Protocols
WB protocol for CSNK1A1 antibody 68434-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols