Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, NIH/3T3 cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human testis tissue, human thyroid tissue, mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
22418-1-AP targets CSNK2B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CSNK2B fusion protein Ag18134 相同性解析による交差性が予測される生物種 |
| Full Name | casein kinase 2, beta polypeptide |
| Calculated molecular weight | 215 aa, 25 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC112017 |
| Gene Symbol | CSNK2B |
| Gene ID (NCBI) | 1460 |
| RRID | AB_2879102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P67870 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK2B antibody 22418-1-AP | Download protocol |
| IHC protocol for CSNK2B antibody 22418-1-AP | Download protocol |
| FC protocol for CSNK2B antibody 22418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells. | ||
Cancer Metab Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1 | ||
Nat Immunol GIMAP5 deficiency reveals a mammalian ceramide-driven longevity assurance pathway |