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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2B Monoclonal antibody (67866-1-Ig)

    WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CSNK2B Monoclonal antibody (67866-1-Ig)

    WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

    WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2B Monoclonal antibody (67866-1-Ig)

    IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CSNK2B Monoclonal antibody, PBS Only

CSNK2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67866-1-PBS
Clone No.1B5A6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67866-1-PBS targets CSNK2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CSNK2B fusion protein Ag19180 相同性解析による交差性が予測される生物種
Full Name casein kinase 2, beta polypeptide
Calculated molecular weight 215 aa, 25 kDa
Observed molecular weight27 kDa
GenBank accession numberBC112017
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
RRIDAB_2918624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP67870
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.