CSPG4 Polyclonal antibody

CSPG4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31623-1-AP

Synonyms

NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP


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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31623-1-AP targets CSPG4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chondroitin sulfate proteoglycan 4
Calculated molecular weight251kd
Observed molecular weight 250-300 kDa
GenBank accession numberNM_001897.5
Gene symbol CSPG4
Gene ID (NCBI) 1464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSPG4 is a proteoglycan that plays a role in cell proliferation and migration, stimulates endothelial cell motility during microvascular morphogenesis and may also inhibit neurite outgrowth and growth cone collapse during axon regeneration. CSPG4 is expressed on more than 90% of human melanoma tissues and cultured cells. It is a target of specific diagnosis and immunotherapy with antiidiotypic antibodies due, in part, to its roles in growth control, adhesion, cell-substratum interactions, and cell-cell contacts (PMID: 10587647; 11278606).

Protocols

Product Specific Protocols
WB protocol for CSPG4 antibody 31623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols