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  • Featured Product
  • KD/KO Validated

CST1 Polyclonal antibody, PBS Only

CST1 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16025-1-PBS

Synonyms

Cystatin-SN, Cystatin-1, cystatin SN, Cystatin 1, Cystain-SA-I

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human saliva using 16025-1-AP (same clone as 16025-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human testis using 16025-1-AP (same clone as 16025-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of COLO 320 using 16025-1-AP (same clone as 16025-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human adrenal gland using 16025-1-AP (same clone as 16025-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using CST1 Polyclonal antibody (16025-1-AP)

    FC experiment of MCF-7 using 16025-1-AP (same clone as 16025-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16025-1-PBS targets CST1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CST1 fusion protein Ag8862 相同性解析による交差性が予測される生物種
Full Name cystatin SN
Calculated molecular weight 141 aa, 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC021225
Gene symbol CST1
Gene ID (NCBI) 1469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.