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KD/KO Validated

CST6 Polyclonal antibody

Name: CST6 Polyclonal antibody
Brand: Proteintech
SKU: 17076-1-AP

CST6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17076-1-AP

Synonyms

CST6, Cystatin 6, Cystatin E, cystatin E/M, Cystatin M

Validation Data Gallery

Filter:

AllIHC

IHC staining of human breast cancer using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse heart using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse liver using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse liver using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17076-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 17076-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected in

human breast cancer tissue, mouse skin tissue, mouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

The immunogen of 17076-1-AP is CST6 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CST6 fusion protein Ag10965 相同性解析による交差性が予測される生物種
Full Name	cystatin E/M
Calculated molecular weight	149 aa, 17 kDa
GenBank accession number	BC031334
Gene symbol	CST6
Gene ID (NCBI)	1474
RRID	AB_2878345
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for CST6 antibody 17076-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Theranostics CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis Authors - Xiaoxun Li KD Validated View Article
human	WB	J Proteome Res Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease. Authors - Yong Zhou View Article
human	IHC	Int J Gen Med Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery Authors - Xiaopeng Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CST6 Polyclonal antibody

CST6 Polyclonal Antibody for IHC, ELISA

Validation Data Gallery

IHC staining of human breast cancer using 17076-1-AP

IHC staining of human breast cancer using 17076-1-AP

IHC staining of mouse skin using 17076-1-AP

IHC staining of mouse skin using 17076-1-AP

IHC staining of mouse skin using 17076-1-AP

IHC staining of mouse skin using 17076-1-AP

IHC staining of mouse heart using 17076-1-AP

IHC staining of mouse heart using 17076-1-AP

IHC staining of mouse liver using 17076-1-AP

IHC staining of mouse liver using 17076-1-AP

IHC staining of human breast cancer using 17076-1-AP

IHC staining of human breast cancer using 17076-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery