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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

Cystatin A Polyclonal antibody

Cystatin A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15962-1-AP

Synonyms

CSTA, Stefin-A, Cystatin-A, N-terminally processed, Cystatin-A, Cystatin AS

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Cystatin A Polyclonal antibody (15962-1-AP)

    WB analysis of human saliva using 15962-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cystatin A Polyclonal antibody (15962-1-AP)

    WB analysis of NIH/3T3 using 15962-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cystatin A Polyclonal antibody (15962-1-AP)

    WB analysis of A549 using 15962-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Cystatin A Polyclonal antibody (15962-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Cystatin A Polyclonal antibody (15962-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cystatin A Polyclonal antibody (15962-1-AP)

    IF Staining of HepG2 using 15962-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cystatin A antibody (15962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman saliva tissue, NIH/3T3 cells, A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15962-1-AP targets Cystatin A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cystatin A fusion protein Ag8733 相同性解析による交差性が予測される生物種
Full Name cystatin A (stefin A)
Calculated molecular weight 98 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC010379
Gene symbol Cystatin A
Gene ID (NCBI) 1475
RRIDAB_10641043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cystatin A antibody 15962-1-APDownload protocol
IHC protocol for Cystatin A antibody 15962-1-APDownload protocol
IF protocol for Cystatin A antibody 15962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Res

Lung cancer diagnosis from proteomic analysis of preinvasive lesions.

Authors - Rahman S M Jamshedur SM
View Article
mouseWB,IF

Biochem Biophys Res Commun

CSTA plays a role in osteoclast formation and bone resorption by mediating the DAP12/TREM2 pathway

Authors - Rui Wei
  • KD Validated
View Article
human,mouseWB

Cell Rep

TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation

Authors - Jihuan Hou
View Article