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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSTF1 Polyclonal antibody, PBS Only

CSTF1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10064-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSTF1 Polyclonal antibody (10064-2-AP)

    WB analysis using 10064-2-AP (same clone as 10064-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10064-2-AP (CSTF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using CSTF1 Polyclonal antibody (10064-2-AP)

    IP experiment of NIH/3T3 using 10064-2-AP (same clone as 10064-2-PBS)

    IP result of anti-CSTF1 (IP:10064-2-AP, 2ug; Detection:10064-2-AP 1:500) with NIH/3T3 cells lysate 2500ug. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10064-2-PBS targets CSTF1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF1 fusion protein Ag0109 相同性解析による交差性が予測される生物種
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Calculated molecular weight 48.3 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001011
Gene symbol CSTF1
Gene ID (NCBI) 1477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.