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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSTF1 Polyclonal antibody (10064-2-AP)

    WB analysis using 10064-2-AP (same clone as 10064-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10064-2-AP (CSTF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using CSTF1 Polyclonal antibody (10064-2-AP)

    IP experiment of NIH/3T3 using 10064-2-AP (same clone as 10064-2-PBS)

    IP result of anti-CSTF1 (IP:10064-2-AP, 2ug; Detection:10064-2-AP 1:500) with NIH/3T3 cells lysate 2500ug. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

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CSTF1 Polyclonal antibody, PBS Only

CSTF1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 10064-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10064-2-PBS targets CSTF1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF1 fusion protein Ag0109 相同性解析による交差性が予測される生物種
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Calculated molecular weight 48.3 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001011
Gene Symbol CSTF1
Gene ID (NCBI) 1477
RRIDAB_2261145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05048
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.