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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSTF2T Polyclonal antibody

CSTF2T Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14206-1-AP

Synonyms

TauCstF-64, TauCstF 64, KIAA0689, CstF 64T, Cleavage stimulation factor 64 kDa subunit tau variant

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSTF2T Polyclonal antibody (14206-1-AP)

    WB analysis using 14206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14206-1-AP (CSTF2T antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CSTF2T Polyclonal antibody (14206-1-AP)

    WB analysis of human brain using 14206-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14206-1-AP (CSTF2T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CSTF2T Polyclonal antibody (14206-1-AP)

    WB analysis of rat brain using 14206-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14206-1-AP (CSTF2T Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CSTF2T Polyclonal antibody (14206-1-AP)

    IP experiment of mouse brain using 14206-1-AP

    IP result of anti-CSTF2T (IP:14206-1-AP, 4ug; Detection:14206-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using CSTF2T Polyclonal antibody (14206-1-AP)

    IP experiment of HeLa using 14206-1-AP

    IP result of anti-CSTF2T (IP:14206-1-AP, 4ug; Detection:14206-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using CSTF2T Polyclonal antibody (14206-1-AP)

    IP experiment of HeLa using 14206-1-AP

    IP result of anti-CSTF2T (IP:14206-1-AP, 4ug; Detection:14206-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CSTF2T Polyclonal antibody (14206-1-AP)

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CSTF2T Polyclonal antibody (14206-1-AP)

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CSTF2T Polyclonal antibody (14206-1-AP)

    IF Staining of HepG2 using 14206-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14206-1-AP (CSTF2T antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, rat brain tissue, human brain tissue, HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14206-1-AP targets CSTF2T in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF2T fusion protein Ag5411 相同性解析による交差性が予測される生物種
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
Calculated molecular weight 64 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC028239
Gene symbol CSTF2T
Gene ID (NCBI) 23283
RRIDAB_2086478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CSTF2T antibody 14206-1-APDownload protocol
IHC protocol for CSTF2T antibody 14206-1-APDownload protocol
IF protocol for CSTF2T antibody 14206-1-APDownload protocol
IP protocol for CSTF2T antibody 14206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols