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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTAGE1 Polyclonal antibody

CTAGE1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13267-1-AP

Synonyms

Cancer/testis antigen 21.2, CT21.1, CT21.2, CT21.2,CTAGE2, CTAGE, CTAGE 1, CTAGE 2, CTAGE1, CTAGE2, Protein cTAGE 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTAGE1 Polyclonal antibody (13267-1-AP)

    WB analysis using 13267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13267-1-AP (CTAGE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CTAGE1 Polyclonal antibody (13267-1-AP)

    IP experiment of HeLa using 13267-1-AP

    IP result of anti-CTAGE1 (IP:13267-1-AP, 4ug; Detection:13267-1-AP 1:500) with HeLa cells lysate 2400ug.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13267-1-AP targets CTAGE1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTAGE1 fusion protein Ag3948 相同性解析による交差性が予測される生物種
Full Name cutaneous T-cell lymphoma-associated antigen 1
Calculated molecular weight 754 aa, 89 kDa
Observed molecular weight 86-120 kDa
GenBank accession numberBC031065
Gene symbol CTAGE1
Gene ID (NCBI) 64693
RRIDAB_2086621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CTAGE1 antibody 13267-1-APDownload protocol
IP protocol for CTAGE1 antibody 13267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
View Article
humanWB

Clin Cancer Res

Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.

Authors - Ivan V Litvinov
View Article