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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTAGE1 Monoclonal antibody, PBS Only

CTAGE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4C2

Cat no : 60087-1-PBS

Synonyms

Cancer/testis antigen 21.2, CT21.1, CT21.2, CT21.2,CTAGE2, CTAGE, CTAGE 1, CTAGE 2, CTAGE1, CTAGE2, Protein cTAGE 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CTAGE1 Monoclonal antibody (60087-1-Ig)

    WB analysis of HeLa using 60087-1-Ig (same clone as 60087-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60087-1-Ig (CTAGE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IHC staining of mouse testis using 60087-1-Ig (same clone as 60087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60087-1-Ig (CTAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IHC staining of mouse testis using 60087-1-Ig (same clone as 60087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60087-1-Ig (CTAGE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60087-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IF Staining of MCF-7 using 60087-1-Ig (same clone as 60087-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using CTAGE1 antibody 60087-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60087-1-PBS targets CTAGE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CTAGE1 fusion protein Ag3948 相同性解析による交差性が予測される生物種
Full Name cutaneous T-cell lymphoma-associated antigen 1
Calculated molecular weight 754 aa, 89 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC031065
Gene symbol CTAGE1
Gene ID (NCBI) 64693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.