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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HEK-293 using 66995-1-Ig (same clone as 66995-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of A549 using 66995-1-Ig (same clone as 66995-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HL-60 using 66995-1-Ig (same clone as 66995-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HSC-T6 using 66995-1-Ig (same clone as 66995-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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CTBP1 Monoclonal antibody, PBS Only

CTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66995-1-PBS
Clone No.1C4B2

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66995-1-PBS targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag28567 相同性解析による交差性が予測される生物種
Full Name C-terminal binding protein 1
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2882312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13363
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.