CTGF Polyclonal antibody
CTGF Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
WB, IHC, IF, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 25474-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, U2OS cells |
| Positive IP detected in | HUVEC cells |
| Positive FC (Intra) detected in | HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 30 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
25474-1-AP targets CTGF in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, canine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | connective tissue growth factor |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | NM_001901 |
| Gene symbol | CTGF |
| Gene ID (NCBI) | 1490 |
| RRID | AB_2918089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTGF antibody 25474-1-AP | Download protocol |
| IP protocol for CTGF antibody 25474-1-AP | Download protocol |
| FC protocol for CTGF antibody 25474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo. | ||
Front Bioeng Biotechnol Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis | ||
Eur J Pharmacol Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway | ||
PLoS Negl Trop Dis Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach | ||
Chin Med Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis. | ||
J Agric Food Chem Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy |
