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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using CTHRC1 Recombinant antibody (83629-1-RR)

    WB analysis of A375 using 83629-1-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 83629-1-RR (CTHRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A375 cells using CTHRC1 Recombinant antibody (83629-1-RR)

    FC experiment of A375 using 83629-1-RR

    1x10^6 A375 cells were intracellularly stained with 0.25 ug CTHRC1 Recombinant antibody (83629-1-RR, Clone:240442E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83629-1-RR

    Affinity and Kinetic Characterization of 83629-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83629-1-RR against Human CTHRC1 were performed. The affinity constant is 72.9 pM.

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CTHRC1 Recombinant antibody

CTHRC1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83629-1-RR
Clone No.240442E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1, 240442E1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells
Positive FC (Intra) detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83629-1-RR targets CTHRC1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CTHRC1 fusion protein Ag9812 相同性解析による交差性が予測される生物種
Full Name collagen triple helix repeat containing 1
Calculated molecular weight 243 aa, 26 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC014245
Gene Symbol CTHRC1
Gene ID (NCBI) 115908
RRIDAB_3671242
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96CG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

Protocols

Product Specific Protocols
WB protocol for CTHRC1 antibody 83629-1-RRDownload protocol
FC protocol for CTHRC1 antibody 83629-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols