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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTLA-4 Polyclonal antibody (30648-1-AP)

    WB analysis using 30648-1-AP

    Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CTLA-4/CD152 Polyclonal antibody (30648-1-AP)

    IF Staining of mouse spleen using 30648-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CTLA-4 antibody (30648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).

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CTLA-4/CD152 Polyclonal antibody

CTLA-4/CD152 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30648-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, ELISA

Synonyms

CTLA-4, Ctla4, Ly 56, Ctla 4, Cd152

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCon A treated mouse splenocytes
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30648-1-AP targets CTLA-4/CD152 in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic T-lymphocyte-associated protein 4
Calculated molecular weight25 kDa
Observed molecular weight28-40 kDa
GenBank accession numberNM_009843.4
Gene Symbol CTLA-4
Gene ID (NCBI) 12477
RRIDAB_3086380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09793
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

Protocols

Product Specific Protocols
WB protocol for CTLA-4/CD152 antibody 30648-1-APDownload protocol
IF protocol for CTLA-4/CD152 antibody 30648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int Immunopharmacol

Chemokine‑ and chemokine receptor-based subtypes predict prognosis, immunotherapy and chemotherapy response in colorectal cancer patients

Authors - Wenjie Zhu
View Article