• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody, PBS Only

CTLA-4/CD152 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241100B8

Cat no : 98147-1-PBS

Synonyms

Ctla4, Ly 56, Ctla 4, Cd152, 241100B8

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibod (98147-1-RR)

    FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    1x10^6 untreated or Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibod (98147-1-RR)

    FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    1x10^6 Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98147-1-RR

    Affinity and Kinetic Characterization of 98147-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98147-1-RR against Mouse CTLA-4/CD152 were performed. The affinity constant is 0.580 nM.

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98147-1-PBS is CTLA-4/CD152 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic T-lymphocyte-associated protein 4
Calculated molecular weight25 kDa
GenBank accession numberNM_009843.4
Gene symbol CTLA-4
Gene ID (NCBI) 12477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).