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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CTNNBL1 Polyclonal antibody (13665-1-AP)

    WB analysis of Jurkat using 13665-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CTNNBL1 Polyclonal antibody (13665-1-AP)

    WB analysis of mouse spleen using 13665-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CTNNBL1 Polyclonal antibody (13665-1-AP)

    WB analysis of mouse thymus using 13665-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CTNNBL1 Polyclonal antibody (13665-1-AP)

    IHC staining of human testis using 13665-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CTNNBL1 Polyclonal antibody (13665-1-AP)

    IHC staining of human testis using 13665-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 10x lens).

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CTNNBL1 Polyclonal antibody

CTNNBL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13665-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Beta catenin like protein 1, C20orf33, catenin, beta like 1, CTNNBL1, NAP, Nuclear associated protein, NYD SP19, P14L, PP8304

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13665-1-AP targets CTNNBL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTNNBL1 fusion protein Ag4578 相同性解析による交差性が予測される生物種
Full Name catenin, beta like 1
Calculated molecular weight 563 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC036739
Gene Symbol CTNNBL1
Gene ID (NCBI) 56259
RRIDAB_2086245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTNNBL1, also named as C20orf33, belongs to the armadillo-containing protein family (PMID: 32484799). CTNNBL1 is a widely expressed nucleoprotein that associates with the pre-mRNA processing factor 19 (Prp19) complex of the spliceosome, interacting with its CDC5L component. CTNNBL1 can maintain the content and integrity of the Prp19 spliceosome complex by regulating the interaction between CWC15 and CDC5L (PMID: 28501461). CTNNBL1 has 4 isoforms with the molecular weights of 36, 43, 62 and 65 kDa, respectively. CTNNBL1 may play an important role in colorectal cancer and regulating AID function (PMID: 28501461, 32484799).

Protocols

Product Specific Protocols
WB protocol for CTNNBL1 antibody 13665-1-APDownload protocol
IHC protocol for CTNNBL1 antibody 13665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Role of Glycated High Mobility Group Box-1 in Gastric Cancer.

Authors - Shingo Kishi
View Article
humanWB

J Transl Med

eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathway.

Authors - Liping Sun
View Article