CTNS Polyclonal antibody

CTNS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13085-1-AP

Synonyms

Cystinosin


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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13085-1-AP targets CTNS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTNS fusion protein Ag3904 相同性解析による交差性が予測される生物種
Full Name cystinosis, nephropathic
Calculated molecular weight400aa,45 kDa; 367aa,42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC032850
Gene symbol CTNS
Gene ID (NCBI) 1497
RRIDAB_2230084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CTNS or cystinosin is a lysosomal membrane protein which comprises with seven transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a cytosolic C-terminal GYDQL sorting motif. It functions as a H+-driven cystine transporter responsible for cystine export from lysosomes. The mutation of CTNS gene cause an inherited disorder, cystinosis, characterized by defective lysosomal efflux of cystine. This antibody was raised against the N-terminal region of human cystinosin. Two isoforms of CTNS exist due to alternative splicing events and both of them can be detected through this antibody.

Protocols

Product Specific Protocols
WB protocol for CTNS antibody 13085-1-APDownload protocol
IF protocol for CTNS antibody 13085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biochem Biophys Res Commun

Reduced phosphate transport in the renal proximal tubule cells in cystinosis is due to decreased expression of transporters rather than an energy defect.

Authors - Mary L Taub
humanWB

Biochem Biophys Res Commun

Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.

Authors - Jong-Jer Lee
mouseIF

J Immunol Methods

Characterization of a rat monoclonal antibody raised against ferroptotic cells.

Authors - Sho Kobayashi