CTPS2 Polyclonal antibody

CTPS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 12852-1-AP

Synonyms

UTP--ammonia ligase 2, UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II


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Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, human adrenal gland tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12852-1-AP targets CTPS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTPS2 fusion protein Ag3490 相同性解析による交差性が予測される生物種
Full Name CTP synthase II
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034986
Gene symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_10639520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Protocols

Product Specific Protocols
WB protocol for CTPS2 antibody 12852-1-APDownload protocol
IHC protocol for CTPS2 antibody 12852-1-APDownload protocol
IP protocol for CTPS2 antibody 12852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int Rev Cell Mol Biol

Molecular cell biology and immunobiology of mammalian rod/ring structures.

Authors - Wendy C Carcamo
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
humanWB

J Genet Genomics

Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.

Authors - Gerson Dierley Keppeke