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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTPS2 Monoclonal antibody (67734-1-Ig)

    WB analysis using 67734-1-Ig (same clone as 67734-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using CTPS2 Monoclonal antibody (67734-1-Ig)

    WB analysis of U2OS using 67734-1-Ig (same clone as 67734-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50097-1

    Sandwich ELISA standard curve of MP50097-1

    Sandwich ELISA standard curve of MP50097-1, CTPS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67734-1-PBS. Detection antibody: 67734-2-PBS. Standard:Ag30258. Range: 0.195-50 ng/mL.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IF Staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CTPS2 Monoclonal antibody (67734-1-Ig)

    IF Staining of PC-12 using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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CTPS2 Monoclonal antibody, PBS Only (Capture)

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 67734-1-PBS
Clone No.1A3B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

Synonyms

UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II, CTP synthase 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67734-1-PBS targets CTPS2 as part of a matched antibody pair:

MP50097-1: 67734-1-PBS capture and 67734-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CTPS2 fusion protein Ag30258 相同性解析による交差性が予測される生物種
Full Name CTP synthase II
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC034986
Gene Symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_2882918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRF8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.