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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTRP1 Polyclonal antibody (30289-1-AP)

    WB analysis using 30289-1-AP

    human adipose tissue was subjected to SDS PAGE followed by western blot with 30289-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CTRP1 Polyclonal antibody (30289-1-AP)

    IHC staining of mouse heart using 30289-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CTRP1 Polyclonal antibody (30289-1-AP)

    IHC staining of mouse kidney using 30289-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CTRP1 Polyclonal antibody

CTRP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30289-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP, UNQ310/PRO353

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman adipose tissue
Positive IHC detected inmouse heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30289-1-AP targets CTRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTRP1 fusion protein Ag31779 相同性解析による交差性が予測される生物種
Full Name C1q and tumor necrosis factor related protein 1
Calculated molecular weight32KD
Observed molecular weight 35 kDa
GenBank accession numberNM_030968
Gene Symbol CTRP1
Gene ID (NCBI) 114897
RRIDAB_3669708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

Protocols

Product Specific Protocols
WB protocol for CTRP1 antibody 30289-1-APDownload protocol
IHC protocol for CTRP1 antibody 30289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols