• Featured Product
  • KD/KO Validated

Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21327-1-AP

Synonyms

CTSD, EC:3.4.23.5, CPSD, CLN10, Cathepsin D light chain


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inA375 cells, HuH-7 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21327-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin D fusion protein Ag15254 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight 412 aa, 45 kDa
Observed molecular weight 32 kDa, 48 kDa, 52 kDa
GenBank accession numberBC016320
Gene symbol Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_10733646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 21327-1-APDownload protocol
IHC protocol for Cathepsin D antibody 21327-1-APDownload protocol
IF protocol for Cathepsin D antibody 21327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
mouseWB

Mol Cell

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

Authors - Zhenxing Zhang
mouseWB

Acta Pharm Sin B

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.

Authors - Xiliang Du
mouseWB,IF

Redox Biol

Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury

Authors - Yu Xu
human,mouseWB,IF

Autophagy

Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.

Authors - M Iqbal Hossain
  • KD Validated
mouseWB

Cell Death Differ

Programmed cell death 4 modulates lysosomal function by inhibiting TFEB translation.

Authors - Xiaotong Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chen (Verified Customer) (06-01-2021)

Very good to detect cathepsin D in postmortem human samples with Western blotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: postmortem human brain lysate
Cathepsin D Antibody Western Blot validation (1:3000 dilution) in postmortem human brain lysate (Cat no:21327-1-AP)
FH

Efil (Verified Customer) (05-29-2019)

cells were fixed in cold MeOH for 8 minutes

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human fibroblasts
Cathepsin D Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:21327-1-AP)