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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cathepsin V Polyclonal antibody

Cathepsin V Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18442-1-AP

Synonyms

CTSL2, CTSV, CTSU, CATL2, Cathepsin U

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin V Polyclonal antibody (18442-1-AP)

    WB analysis using 18442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Cathepsin V Polyclonal antibody (18442-1-AP)

    WB analysis of human testis using 18442-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Cathepsin V Polyclonal antibody (18442-1-AP)

    WB analysis of mouse testis using 18442-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cathepsin V Polyclonal antibody (18442-1-AP)

    IHC staining of human skin cancer using 18442-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cathepsin V Polyclonal antibody (18442-1-AP)

    IHC staining of human skin cancer using 18442-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

18442-1-AP targets Cathepsin V in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin V fusion protein Ag13284 相同性解析による交差性が予測される生物種
Full Name cathepsin L2
Calculated molecular weight 37 kDa
Observed molecular weight26-36 kDa
GenBank accession numberBC023504
Gene symbol Cathepsin V
Gene ID (NCBI) 1515
RRIDAB_10644340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Protocols

Product Specific Protocols
WB protocol for Cathepsin V antibody 18442-1-APDownload protocol
IHC protocol for Cathepsin V antibody 18442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Carcinog

Cathepsin V is correlated with the prognosis and tumor microenvironment in liver cancer

Authors - Junyu Liu
View Article