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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTSO Polyclonal antibody

CTSO Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14296-1-AP

Synonyms

cathepsin O, CTSO, CTSO1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using CTSO Polyclonal antibody (14296-1-AP)

    IP experiment of HEK-293 using 14296-1-AP

    IP result of anti-CTSO (IP:14296-1-AP, 4ug; Detection:14296-1-AP 1:300) with HEK-293 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CTSO Polyclonal antibody (14296-1-AP)

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CTSO Polyclonal antibody (14296-1-AP)

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14296-1-AP targets CTSO in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTSO fusion protein Ag5651 相同性解析による交差性が予測される生物種
Full Name cathepsin O
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC049206
Gene symbol CTSO
Gene ID (NCBI) 1519
RRIDAB_2878042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CTSO antibody 14296-1-APDownload protocol
IP protocol for CTSO antibody 14296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols