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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTSS Polyclonal antibody

CTSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27538-1-AP

Synonyms

EC:3.4.22.27, Cathepsin S

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTSS Polyclonal antibody (27538-1-AP)

    WB analysis using 27538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (CTSS antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CTSS Polyclonal antibody (27538-1-AP)

    WB analysis using 27538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (CTSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CTSS Polyclonal antibody (27538-1-AP)

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CTSS Polyclonal antibody (27538-1-AP)

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using CTSS Polyclonal antibody (27538-1-AP)

    IF Staining of U-87 MG using 27538-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CTSS antibody (27538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHUVEC cells, THP-1 cells, U-87 MG cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27538-1-AP targets CTSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTSS fusion protein Ag25881 相同性解析による交差性が予測される生物種
Full Name cathepsin S
Calculated molecular weight 37 kDa
Observed molecular weight 25 kDa, 37 kDa
GenBank accession numberBC002642
Gene symbol CTSS
Gene ID (NCBI) 1520
RRIDAB_3085966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cathepsin S (CTSS) is a cysteine protease that plays an important role in extracellular matrix degradation and remodeling, antigen presentation, inflammation, and angiogenesis; it has been identified as a radiation response gene. In malignant tumors, CTSS induces tumor proliferation, invasion and metastasis through various mechanisms. CTSS proteins could be detected in two forms, pro-CTSS (35-37 kDa) and active CTSS (25 kDa) (PMID: 30006610, PMID: 34208434). And in this publication, it was also reporeted that active CTSS was expressed at higher levels than pro-CTSS in the lysate (PMID: 34208434).

Protocols

Product Specific Protocols
WB protocol for CTSS antibody 27538-1-APDownload protocol
IHC protocol for CTSS antibody 27538-1-APDownload protocol
IF protocol for CTSS antibody 27538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols