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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTTNBP2NL Polyclonal antibody

CTTNBP2NL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25523-1-AP

Synonyms

KIAA1433, CTTNBP2 N-terminal-like protein, CTTNBP2 N terminal like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    WB analysis using 25523-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    WB analysis of mouse skin using 25523-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    WB analysis of MDA-MB-453s using 25523-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    IP experiment of MCF-7 using 25523-1-AP

    IP result of anti-CTTNBP2NL (IP:25523-1-AP, 4ug; Detection:25523-1-AP 1:600) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    IHC staining of human ovary cancer using 25523-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    IF Staining of MCF-7 using 25523-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CTTNBP2NL Polyclonal antibody (25523-1-AP)

    IF Staining of U2OS using 25523-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MDA-MB-453s cells, mouse skin tissue, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25523-1-AP targets CTTNBP2NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTTNBP2NL fusion protein Ag22137 相同性解析による交差性が予測される生物種
Full Name CTTNBP2 N-terminal like
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC016029
Gene symbol CTTNBP2NL
Gene ID (NCBI) 55917
RRIDAB_2880117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CTTNBP2NL antibody 25523-1-APDownload protocol
IHC protocol for CTTNBP2NL antibody 25523-1-APDownload protocol
IF protocol for CTTNBP2NL antibody 25523-1-APDownload protocol
IP protocol for CTTNBP2NL antibody 25523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed
View Article