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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CUEDC2 Polyclonal antibody

CUEDC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20123-1-AP

Synonyms

HOYS6, CUE domain-containing protein 2, CUE domain containing 2, C10orf66

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis using 20123-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of mouse kidney using 20123-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of human brain using 20123-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of human kidney using 20123-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IP experiment of mouse brain using 20123-1-AP

    IP result of anti-CUEDC2 (IP:20123-1-AP, 4ug; Detection:20123-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human ovary tumor using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human ovary tumor using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human breast cancer using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human breast cancer using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human malignant melanoma using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human malignant melanoma using 20123-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUEDC2 Polyclonal antibody (20123-1-AP)

    IF Staining of HeLa using 20123-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUEDC2 antibody (20123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHepG2 cells, mouse kidney tissue, HeLa cells, human brain tissue, human kidney tissue, Jurkat cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20123-1-AP targets CUEDC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUEDC2 fusion protein Ag13847 相同性解析による交差性が予測される生物種
Full Name CUE domain containing 2
Calculated molecular weight 287 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000262
Gene symbol CUEDC2
Gene ID (NCBI) 79004
RRIDAB_10638318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.

Protocols

Product Specific Protocols
WB protocol for CUEDC2 antibody 20123-1-APDownload protocol
IHC protocol for CUEDC2 antibody 20123-1-APDownload protocol
IF protocol for CUEDC2 antibody 20123-1-APDownload protocol
IP protocol for CUEDC2 antibody 20123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols