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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28404-1-AP

Synonyms

CUL 1, CUL1, cullin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL1 Polyclonal antibody (28404-1-AP)

    WB analysis using 28404-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28404-1-AP (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL1 Polyclonal antibody (28404-1-AP)

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL1 Polyclonal antibody (28404-1-AP)

    IHC staining of human breast cancer using 28404-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28404-1-AP (CUL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28404-1-AP targets CUL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL1 fusion protein Ag29145 相同性解析による交差性が予測される生物種
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
RRIDAB_2881135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 28404-1-APDownload protocol
IHC protocol for CUL1 antibody 28404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols