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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CUL1 Monoclonal antibody

CUL1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B6D10

Cat no : 66978-1-Ig

Synonyms

Cullin-1, Cullin1, CUL-1, 1B6D10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL1 Monoclonal antibody (66978-1-Ig)

    WB analysis using 66978-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66978-1-Ig (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using CUL1 Monoclonal antibody (66978-1-Ig)

    WB analysis using 66978-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66978-1-Ig (CUL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CUL1 Monoclonal antibody (66978-1-Ig)

    IHC staining of human lung cancer using 66978-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66978-1-Ig (CUL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL1 Monoclonal antibody (66978-1-Ig)

    IHC staining of human prostate cancer using 66978-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66978-1-Ig (CUL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CUL1 Monoclonal antibody (66978-1-Ig)

    IF Staining of HEK-293 using 66978-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CUL1 antibody (66978-1-Ig, Clone: 1B6D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using CUL1 Monoclonal antibody (66978-1-Ig)

    FC experiment of HEK-293 using 66978-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (66978-1-Ig, Clone:1B6D10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, NCI-H1299 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66978-1-Ig targets CUL1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CUL1 fusion protein Ag28399 相同性解析による交差性が予測される生物種
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
RRIDAB_2882298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 66978-1-IgDownload protocol
IHC protocol for CUL1 antibody 66978-1-IgDownload protocol
IF protocol for CUL1 antibody 66978-1-IgDownload protocol
FC protocol for CUL1 antibody 66978-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols