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CUL1 Recombinant antibody

CUL1 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5K17

Cat no : 82474-1-RR

Synonyms

Cullin-1, Cullin1, CUL-1, 5K17

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CUL1 Recombinant antibody (82474-1-RR)

    WB analysis of A549 using 82474-1-RR

    WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using CUL1 Recombinant antibody (82474-1-RR)

    WB analysis using 82474-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CUL1 Recombinant antibody (82474-1-RR)

    IP experiment of HeLa using 82474-1-RR

    IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug.

  • Affinity and Kinetic Characterization of 82474-1-RR

    Affinity and Kinetic Characterization of 82474-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:17000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82474-1-RR targets CUL1 in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CUL1 fusion protein Ag3585 相同性解析による交差性が予測される生物種
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 82474-1-RRDownload protocol
IP protocol for CUL1 antibody 82474-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols