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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CUL2 Polyclonal antibody

CUL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30071-1-AP

Synonyms

CUL 2, CUL2, cullin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL2 Polyclonal antibody (30071-1-AP)

    WB analysis using 30071-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30071-1-AP (CUL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CUL2 Polyclonal antibody (30071-1-AP)

    WB analysis of PC-3 using 30071-1-AP

    WB result of CUL2 antibody (30071-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected PC-3 cells.

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Tested Applications

Positive WB detected inPC-3 cells, Raji cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30071-1-AP targets CUL2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL2 fusion protein Ag32331 相同性解析による交差性が予測される生物種
Full Name cullin 2
Calculated molecular weight 87 kDa
Observed molecular weight 80-87 kDa
GenBank accession numberBC009591
Gene symbol CUL2
Gene ID (NCBI) 8453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL2 antibody 30071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols