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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis of HeLa using 66038-1-Ig (same clone as 66038-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    WB analysis of pig brain using 66038-1-Ig (same clone as 66038-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CUL4A Monoclonal antibody (66038-1-Ig)

    IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL4A Monoclonal antibody (66038-1-Ig)

    IHC staining of human breast cancer using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66038-1-Ig(CUL4A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CUL4A Monoclonal antibody (66038-1-Ig)

    IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CUL4A Monoclonal antibody, PBS Only

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66038-1-PBS
Clone No.1A7F12

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CUL 4A, CUL4A, cullin 4A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66038-1-PBS targets CUL4A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, monkey, mouse, rat, pig samples.

Tested Reactivity human, monkey, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL4A fusion protein Ag18089 相同性解析による交差性が予測される生物種
Full Name cullin 4A
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa, 88 kDa
GenBank accession numberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
RRIDAB_11042446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13619
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.