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- KD/KO Validated
CUL4B Polyclonal antibody
CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 12916-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 29 publications below |
WB | See 73 publications below |
IHC | See 4 publications below |
IF | See 8 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
RIP | See 1 publications below |
Product Information
12916-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CUL4B fusion protein Ag3563 相同性解析による交差性が予測される生物種 |
Full Name | cullin 4B |
Calculated molecular weight | 913 aa, 104 kDa |
Observed molecular weight | 102 kDa |
GenBank accession number | BC036216 |
Gene symbol | CUL4B |
Gene ID (NCBI) | 8450 |
RRID | AB_2086699 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).
Protocols
Product Specific Protocols | |
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WB protocol for CUL4B antibody 12916-1-AP | Download protocol |
IF protocol for CUL4B antibody 12916-1-AP | Download protocol |
IP protocol for CUL4B antibody 12916-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
| ||
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
Science CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins. | ||
Nat Med Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.
| ||
Science Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. |