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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL4B Monoclonal antibody (60151-1-Ig)

    WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL4B Monoclonal antibody (60151-1-Ig)

    WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL4B Monoclonal antibody (60151-1-Ig)

    WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CUL4B Monoclonal antibody (60151-1-Ig)

    WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CUL4B Monoclonal antibody (60151-1-Ig)

    WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Monoclonal antibody (60151-1-Ig)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CUL4B Monoclonal antibody (60151-1-Ig)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CUL4B Monoclonal antibody, PBS Only

CUL4B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60151-1-PBS
Clone No.4E10B10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60151-1-PBS targets CUL4B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL4B fusion protein Ag3563 相同性解析による交差性が予測される生物種
Full Name cullin 4B
Calculated molecular weight 913 aa, 104 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_10641034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13620
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).