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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUL4B Recombinant antibody (85001-4-RR)

    IF Staining of HeLa using 85001-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85001-4-RR

    Affinity and Kinetic Characterization of 85001-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

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CUL4B Recombinant antibody

CUL4B Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 85001-4-RR
Clone No.242626F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA0695, Cullin-4B, cullin 4B, 242626F11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85001-4-RR targets CUL4B in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CUL4B fusion protein Ag3563 相同性解析による交差性が予測される生物種
Full Name cullin 4B
Calculated molecular weight 913 aa, 104 kDa
GenBank accession numberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Protocols

Product Specific Protocols
IF protocol for CUL4B antibody 85001-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols