Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, LNCaP cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | MCF-7 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83869-1-RR targets Cullin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cullin 5 fusion protein Ag34620 相同性解析による交差性が予測される生物種 |
| Full Name | cullin 5 |
| Calculated molecular weight | 91 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC063306 |
| Gene Symbol | Cullin 5 |
| Gene ID (NCBI) | 8065 |
| RRID | AB_3671455 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q93034 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cullin 5 antibody 83869-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |