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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cullin 5 Recombinant antibody

Cullin 5 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240737A12

Cat no : 83869-1-RR

Synonyms

CUL5, VACM 1, Cullin-5, CUL-5, CUL 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUL5 Recombinant antibody (83869-1-RR)

    WB analysis using 83869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83869-1-RR (CUL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-2 OS using Cullin 5 Recombinant antibody (83869-1-RR)

    FC experiment of U-2 OS using 83869-1-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of MCF-7 cells using Cullin 5 Recombinant antibody (83869-1-RR)

    FC experiment of MCF-7 using 83869-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, LNCaP cells, NIH/3T3 cells
Positive FC (Intra) detected inMCF-7 cells, U-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83869-1-RR is Cullin 5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cullin 5 fusion protein Ag34620 相同性解析による交差性が予測される生物種
Full Name cullin 5
Calculated molecular weight 91 kDa
Observed molecular weight80 kDa
GenBank accession numberBC063306
Gene symbol Cullin 5
Gene ID (NCBI) 8065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).

Protocols

Product Specific Protocols
WB protocol for Cullin 5 antibody 83869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols