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CUX1 Polyclonal antibody

Name: CUX1 Polyclonal antibody
Brand: Proteintech
SKU: 11733-1-AP
Rating: 4.00 (4 reviews)

CUX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (4)

Cat no : 11733-1-AP

Synonyms

CASP, CCAAT displacement protein, CDP, CDP/Cut, CDP1, Clox, COY1, cut like homeobox 1, CUTL1, CUX, Cux/CDP, CUX1, GOLIM6, Homeobox protein cut like 1, Homeobox protein cux 1, Nbla10317, p100, p110, p200, p75

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis using 11733-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11733-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11733-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11733-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11733-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11733-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11733-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11733-1-AP
IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11733-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cortex using 11733-1-AP
IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. .

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Cat No. 11733-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, human brain tissue, human liver tissue, mouse liver tissue, rat brain tissue, mouse brain tissue, L02 cells, rat liver tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse cortex

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)	IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 14 publications below
IHC	See 18 publications below
IF	See 33 publications below
IP	See 1 publications below

Product Information

11733-1-AP targets CUX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CUX1 fusion protein Ag2335 相同性解析による交差性が予測される生物種
Full Name	cut-like homeobox 1
Calculated molecular weight	676 aa, 77 kDa
Observed molecular weight	80 kDa
GenBank accession number	BC025422
Gene symbol	CUX1
Gene ID (NCBI)	1523
RRID	AB_2086995
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).

Protocols

Product Specific Protocols
WB protocol for CUX1 antibody 11733-1-AP	Download protocol
IHC protocol for CUX1 antibody 11733-1-AP	Download protocol
IP protocol for CUX1 antibody 11733-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nature Reversing a model of Parkinson's disease with in situ converted nigral neurons. Authors - Hao Qian View Article
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human	WB	Nat Genet Inactivating CUX1 mutations promote tumorigenesis. Authors - Chi C Wong KD Validated View Article
mouse	IF	Sci Adv IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype. Authors - Yasufumi Hayano View Article
human,mouse	WB	Nat Commun Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Authors - Emmanuelle Supper KD Validated View Article
mouse	IHC	Dev Cell Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development Authors - Xue-Lian Sun View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Reyes (Verified Customer) (04-22-2024)	I tested Cux1 in human brain FFPE tissue and it stained nicely, although the layers are not really sharp Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Human Brain Cortex CUX1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:11733-1-AP)
FH Boyan (Verified Customer) (04-17-2023)	very good for WB Applications: Western Blot
FH Etienne (Verified Customer) (12-19-2022)	Worked once with some noisy staining at 1/200. Didn't work at any other dilution Applications: Immunofluorescence Primary Antibody Dilution: 1/10,1/20,1/100,1/200 Cell Tissue Type: Human Brain Organoids CUX1 Antibody Immunofluorescence validation (1/10,1/20,1/100,1/200 dilution) in Human Brain Organoids (Cat no:11733-1-AP)
FH Carly (Verified Customer) (09-27-2019)	Used in an antibody array to identify proteins in human EDTA plasma. Applications: Other, Cell Tissue Type: Human EDTA Plasma

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CUX1 Polyclonal antibody

CUX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis using 11733-1-AP

WB analysis of human brain using 11733-1-AP

WB analysis of human liver using 11733-1-AP

WB analysis of Jurkat using 11733-1-AP

WB analysis of mouse liver using 11733-1-AP

WB analysis of rat brain using 11733-1-AP

WB analysis of mouse brain using 11733-1-AP

IP experiment of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IHC staining of mouse brain using 11733-1-AP

IF Staining of mouse cortex using 11733-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Inactivating CUX1 mutations promote tumorigenesis.

IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype.

Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia.

Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development

Reviews