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  • Featured Product
  • KD/KO Validated

CUX2 Recombinant antibody

CUX2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230235G1

Cat no : 82933-1-RR

Synonyms

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUX2 Recombinant antibody (82933-1-RR)

    WB analysis using 82933-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CUX2 Recombinant antibody (82933-1-RR)

    WB analysis of SH-SY5Y using 82933-1-RR

    WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUX2 Recombinant antibody (82933-1-RR)

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUX2 Recombinant antibody (82933-1-RR)

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82933-1-RR

    Affinity and Kinetic Characterization of 82933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

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Tested Applications

Positive WB detected inK-562 cells, SH-SY5Y cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82933-1-RR targets CUX2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CUX2 fusion protein Ag18809 相同性解析による交差性が予測される生物種
Full Name cut-like homeobox 2
Calculated molecular weight 1486 aa, 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberBC151245
Gene symbol CUX2
Gene ID (NCBI) 23316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.

Protocols

Product Specific Protocols
WB protocol for CUX2 antibody 82933-1-RRDownload protocol
IF protocol for CUX2 antibody 82933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols