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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using CX3CL1 Polyclonal antibody (10108-2-AP)

    WB analysis of PC-3 using 10108-2-AP (same clone as 10108-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CX3CL1 Polyclonal antibody (10108-2-AP)

    WB analysis of human brain using 10108-2-AP (same clone as 10108-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CX3CL1 Polyclonal antibody (10108-2-AP)

    IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens).. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CX3CL1 Polyclonal antibody (10108-2-AP)

    IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens).. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CX3CL1 Polyclonal antibody (10108-2-AP)

    IHC staining of human lung using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CX3CL1 Polyclonal antibody (10108-2-AP)

    IHC staining of human lung using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10108-2-PBS targets CX3CL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CX3CL1 fusion protein Ag0161 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) ligand 1
Calculated molecular weight 42 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001163
Gene Symbol CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2087154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78423
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75 kDa precursor and undergoes glycosylation to yield two mature forms: a 100 kDa cell membrane bound form and an 85 kDa soluble form which is released from the cell surface. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.