Validation Data Gallery

Filter: AllWBIP

WB analysis of HEK-293 using 13885-1-AP
WB result of CX3CR1 antibody (13885-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CX3CR1 transfected HEK-293 cells.
WB analysis using 13885-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13885-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 13885-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13885-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 13885-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 13885-1-AP
IP result of anti-CX3CR1 (IP:13885-1-AP, 4ug; Detection:13885-1-AP 1:1000) with SH-SY5Y cells lysate 1600ug.

Featured Product
KD/KO Validated

CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13885-1-AP

Publications(39)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA, Cell treatment

Synonyms

CMK BRL1, CMK BRL 1, CCRL1, C X3 C CKR 1, Beta chemokine receptor-like 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (39)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, HEK-293 cells, SH-SY5Y cells, U-937 cells, K-562 cells
Positive IP detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 27 publications below
IHC	See 15 publications below
IF	See 9 publications below
IP	See 1 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

13885-1-AP targets CX3CR1 in WB, IHC, IF, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CX3CR1 fusion protein Ag4149 相同性解析による交差性が予測される生物種
Full Name	chemokine (C-X3-C motif) receptor 1
Calculated molecular weight	40 kDa
Observed molecular weight	44-47 kDa
GenBank accession number	BC028078
Gene Symbol	CX3CR1
Gene ID (NCBI)	1524
RRID	AB_2087418
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49238
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Protocols

Product Specific Protocols
WB protocol for CX3CR1 antibody 13885-1-AP	Download protocol
IP protocol for CX3CR1 antibody 13885-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IP	Brain Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2. Authors - Yun Xia View Article
human	WB,IF	Tissue Cell Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems Authors - Mayuko Takata View Article
mouse	WB	J Control Release Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme. Authors - Sai Qiao View Article
mouse	IHC	Front Immunol Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis Authors - Zi Yang View Article
mouse	WB	Nanoscale Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles. Authors - Liang Ding View Article
human	WB	Mol Oncol Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis. Authors - Shuo Miao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 13885-1-AP

WB analysis using 13885-1-AP

WB analysis of SH-SY5Y using 13885-1-AP

WB analysis of U-937 using 13885-1-AP

WB analysis of K-562 using 13885-1-AP

WB analysis of HL-60 using 13885-1-AP

IP experiment of SH-SY5Y using 13885-1-AP

CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.

Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems

Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.

Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis

Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.

Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.