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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CX3CR1 Polyclonal antibody (29819-1-AP)

    WB analysis using 29819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29819-1-AP (CX3CR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CX3CR1 Polyclonal antibody (29819-1-AP)

    IF Staining of mouse kidney using 29819-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (29819-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 29819-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Beta chemokine receptor like 1, Beta chemokine receptor-like 1, C X3 C CKR 1, CCRL1, CMK BRL 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, THP-1 cells, Caco-2 cells
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29819-1-AP targets CX3CR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CX3CR1 fusion protein Ag27698 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) receptor 1
Calculated molecular weight 40 kDa
Observed molecular weight40 kDa, 44 kDa
GenBank accession numberBC028078
Gene Symbol CX3CR1
Gene ID (NCBI) 1524
RRIDAB_3086173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12). The two CX3CR1 bands exhibited can be attributed to various factors (PMID: 30628679).

Protocols

Product Specific Protocols
WB protocol for CX3CR1 antibody 29819-1-APDownload protocol
IF protocol for CX3CR1 antibody 29819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Control Release

Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720

Authors - Jiaman Xie
View Article