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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CX3CR1 Monoclonal antibody

CX3CR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D3B8

Cat no : 66915-1-Ig

Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CX3CR1 Monoclonal antibody (66915-1-Ig)

    WB analysis using 66915-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CX3CR1 Monoclonal antibody (66915-1-Ig)

    WB analysis of Jurkat using 66915-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CX3CR1 Monoclonal antibody (66915-1-Ig)

    IHC staining of mouse kidney using 66915-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66915-1-Ig (CX3CR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CX3CR1 Monoclonal antibody (66915-1-Ig)

    IF Staining of mouse kidney using 66915-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (66915-1-Ig, Clone: 3D3B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, SH-SY5Y cells, U-937 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66915-1-Ig targets CX3CR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CX3CR1 fusion protein Ag27698 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X3-C motif) receptor 1
Calculated molecular weight 40 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC028078
Gene symbol CX3CR1
Gene ID (NCBI) 1524
RRIDAB_2882242
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CX3CR1, also known as GPR13, V28, and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed in microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as a coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Protocols

Product Specific Protocols
WB protocol for CX3CR1 antibody 66915-1-IgDownload protocol
IHC protocol for CX3CR1 antibody 66915-1-IgDownload protocol
IF protocol for CX3CR1 antibody 66915-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols