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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCL16 Polyclonal antibody (29589-1-AP)

    WB analysis using 29589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29589-1-AP (CXCL16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CXCL16 Polyclonal antibody (29589-1-AP)

    WB analysis using 29589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29589-1-AP (CXCL16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CXCL16 Polyclonal antibody

CXCL16 Polyclonal Antibody for WB, ELISA
Cat No. 29589-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29589-1-AP targets CXCL16 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL16 fusion protein Ag30981 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 16
Calculated molecular weight 273 aa, 30 kDa
Observed molecular weight28 kDa , 50-55 kDa
GenBank accession numberBC017588
Gene Symbol CXCL16
Gene ID (NCBI) 58191
RRIDAB_3086142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2A7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCL16 was discovered as a ligand for CXC chemokine receptor (CXCR) 6. CXCL16 is a membrane-bound chemokine that consists of four distinct domains: the chemokine domain, mucin-like domain, transmembrane domain and ad cytoplasmic. After cleavage, soluble CXCL16 acts as a chemoattractant for activated CD8 T cells, NKT cells and Th1-polarized T cells that express CXCR6. CXCL16 expression has been studied mainly in macrophages and dendritic cells. CXCL16 production by non-immune cells remains controversial. CXCL16 was expressed as two different forms: a 50 kDa form and a 28 kDa form, and the 50 kDa may be the glycosylated form (PMID: 17855433, 33552057). (PMID: 17855433, 33552057).

Protocols

Product Specific Protocols
WB protocol for CXCL16 antibody 29589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols