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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCL3/GRO Gamma Polyclonal antibody (11221-1-AP)

    WB analysis using 11221-1-AP

    Non-treated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 11221-1-AP (CXCL3/GRO Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CXCL3/GRO Gamma Polyclonal antibody

CXCL3/GRO Gamma Polyclonal Antibody for WB, ELISA
Cat No. 11221-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

C X C motif chemokine 3, CINC 2b, CXCL/GRO Gamma, CXCL3, GRO gamma, GRO gamma(1 73), GRO3, GROg, Growth regulated protein gamma, MIP 2b, MIP2 beta, MIP2B, SCYB3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLPS and Protein transport inhibitor treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

11221-1-AP targets CXCL3/GRO Gamma in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL3/GRO Gamma fusion protein Ag1732 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 3
Calculated molecular weight 11 kDa
Observed molecular weight8-10 kDa
GenBank accession numberBC016308
Gene Symbol CXCL3
Gene ID (NCBI) 2921
RRIDAB_3669124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19876
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXC chemokine ligand 3 (CXCL3) is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression.

Protocols

Product Specific Protocols
WB protocol for CXCL3/GRO Gamma antibody 11221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Authors - Zhaohui Chen
View Article