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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIX/CXCL5 Polyclonal antibody

LIX/CXCL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10809-1-AP

Synonyms

CXCL5, ENA-78(1-78), CXCL-5, CXCL 5, C-X-C motif chemokine 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIX/CXCL5 Polyclonal antibody (10809-1-AP)

    WB analysis using 10809-1-AP

    Untreated, PMA treated A549 cells were subjected to SDS PAGE followed by western blot with 10809-1-AP (CXCL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CXCL5 Polyclonal antibody (10809-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10809-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10809-1-AP (CXCL5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPMA treated A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10809-1-AP targets LIX/CXCL5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIX/CXCL5 fusion protein Ag1079 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 5
Calculated molecular weight 12 kDa
Observed molecular weight8 kDa
GenBank accession numberBC008376
Gene symbol CXCL5
Gene ID (NCBI) 6374
RRIDAB_2292366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.

Protocols

Product Specific Protocols
WB protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
IHC protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Stem Cells

Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs: Insights from Single-Cell RNA Sequencing

Authors - Peng Huang
View Article